Coding

Part:BBa_K5091009:Design

Designed by: Angelina Oblak   Group: iGEM24_ULethbridge   (2024-09-30)


pmsB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 91
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 91
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 91
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 91
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing pmsB for use in salicylic acid production, several considerations are important. Co-expression with pmsC in a single operon with an appropriate promoter and RBS for each gene can streamline the process, but balancing expression levels may require tuning. An inducible promoter can help control expression and avoid stress on the host organism. Choosing a low to medium copy number plasmid reduces metabolic burden, and codon optimization is necessary if using a host organism other than Pseudomonas fluorescens. Additionally, monitoring salicylic acid production is crucial to prevent overaccumulation, which could negatively impact the host or target plants.


Source

Pseudomonas fluorescens A506, sequence found through Genebank https://www.ncbi.nlm.nih.gov/gene/12961199

References

Pelludat C, Brem D, Heesemann J. Irp9, encoded by the high-pathogenicity island of Yersinia enterocolitica, is able to convert chorismate into salicylate, the precursor of the siderophore yersiniabactin. J Bacteriol. 2003 Sep;185(18):5648-53. doi: 10.1128/JB.185.18.5648-5653.2003. PMID: 12949119; PMCID: PMC193746.